The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
β-1, 4-MANNANASES FROM MARINE BACTERIA, VIBRIO SPP. MA-129 AND MA-138
TOSHIYOSHI ARAKIYUTAKA TAMARUTATSUO MORISHITA
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1992 Volume 38 Issue 4 Pages 343-351

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Abstract

Two marine bacteria capable of producing extracellular β-1, 4-mannanases (strain nos. MA-129 and MA-138) were isolated from sea environment. A large amount of β-mannanases were induced with konjac powder from both strains and also with mannose from strain no. MA-138. Both strains showed high enzyme production when incubated with shaking at 25°C for 24h. The culture fluid of strain no. MA-129 was fractionated into one active peak and that of strain no. MA-138 two active peaks by ion-exchange chromatography. Every enzyme fraction had pH optimum of 7.0-7.5 and hydrolyzed β-mannan and glucomannan to form several oligosaccharides. Both strains belonged to genus Vibrio.

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