PRODUCTION OF BACTERIOLYTIC ENZYMES DURING THE GROWTH OF A MARINE BACTERIUM ALTEROMONAS SP. No. 8-R

Abstract

Profiles of bacteriolytic enzyme production during the growth of Alteromonas sp. No. 8-R in nutrient broth were investigated by using polyacrylamide gel electrophoresis (PAGE) containing cells of Micrococcus luteus without SDS-treatment. Four bacteriolytic bands (a, b, c, and d) were detected in the culture supernatant and the cell at different stage of growth. Bacteriolytic bands a and c were detected in the culture supernatant in the decreasing phase. Band b was maintained in intracellular to the stationary phase and detected in the culture supernatant at the decreasing phase. Band d was detected in the supernatant from the logarithmic phase to the stationary phase and disappeared at the decreasing phase. The results suggest that the strain has at least four bacteriolytic enzymes and produces enzymes according to different processes during the growth in the nutrient broth.