USE OF MOLECULAR KARYOTYPING FOR DIFFERENTIATION OF SPECIES IN THE HETEROGENEOUS TAXON SACCHAROMYCES EXIGUUS

Abstract

A molecular karyotyping technique consisting of pulsed-field gel electrophoresis of intact chromosomal DNAs and following Southern hybridization with total HindIII-digested DNAs was used as a tool for yeast classification and identification. Significant differences in chromosomal patterns were found among 16 Saccharomyces exiguus strains. The complex taxon, classified into several groups of different karyotypes, showed a low degree of cross-DNA homology.