The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Regulation of lactate dehydrogenase synthesis in a ruminal bacterium, Streptococcus bovis
Narito AsanumaMiwa IwamotoTsuneo Hino
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1997 Volume 43 Issue 6 Pages 325-331


Lactate dehydrogenase (LDH) was purified from three strains of Streptococcus bovis, and the gene Idh was cloned and sequenced. The Idh of S. bovis from a goat (TH1) was different from the Idhs of two other strains from cattle (TH2, JB1) in that Asp220 was substituted for Glu. Northern blot analysis revealed that the LDH-mRNA of S. bovis was approximately 1.0kbp, which was transcribed in amonocistronic fashion. When cells were grown at pH 6.9 in a batch culture, the level of Idh transcript decreased as the growth phase changed; from exponential growth to the cessation of growth. The level of Idh transcript was higher in cells grown at pH 4.5 than at pH 6.9. This observation was consistent with the amounts of LDH in cells and the percentages of lactate produced. These results support the hypothesis that S. bovis regulates LDH synthesis at the transcriptional level probably in response to intracellular pH.

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