The Journal of General and Applied Microbiology
Online ISSN : 1349-8037
Print ISSN : 0022-1260
ISSN-L : 0022-1260
Experimental pathogenicity of Erwinia aphidicola to pea aphid, Acyrthosiphon pisum
Hosami HaradaHajime Ishikawa
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1997 Volume 43 Issue 6 Pages 363-367


Pea aphids were reared aseptically in the laboratory and fed on strains of Erwinia aphidicola, Erwinia herbicola, Klebsiella pneumoniae, Escherichia coli and bacterium W, an aphid enterobacterium, by mixing with a synthetic diet to determine whether these bacteria have pathogenicity to the insect. It turned out that Er. Aphidicola and Er. Herbicola grew in aphid gut, while K. pneumoniae, Es. Coli and bacterium W were effectively eliminated. In the case of Er. aphidicola, ingestion of a single dose of 103CFU/ml was enough to infect aphid gut and to prevent post-final ecdysis growth of the insect. As a result, the body weight of the infected aphids was significantly reduced (p<0.01). A concentration of 105CFU/ml of Er. aphidicola caused aphid mortality. In contrast, Er. herbicola demanded 105CFU/ml to colonize in aphid gut, and seemed to have no effect on insect health.

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