1961 Volume 7 Issue 1 Pages 30-40
1) Brevibacterium flavum, N. 2247 could grow in the chemically defined medium and accumulated a considerable amount of L-glutamic acid in its growing culture medium.
2) It is likely that the effects of biotin on the cell growth and on the L-glutamate accumulation in the acetate medium are not so strict as shown in the glucose medium.
3) aspartic acid, glycine, succinic acid, citric acid and α-keto-glutaric acid were identified as by-products in the broth filtrate.