MICROBIOLOGICAL HYDROXYLATION OF STEROIDS XIII.
OXIDATION OF STEROIDS BY BACILLUS PULVIFACIENS
1961 Volume 7 Issue 2 Pages 118-127
Details
1961 Volume 7 Issue 2 Pages 118-127
1. A strain of microörganism, Bacillus pulvifaciens IAM N-19-2, isolated and identified in this laboratory, was used for oxidative fermentation of various steroids and corresponding 1-dehydro compounds were obtained.
2. Oxidative fermentation of 4-ene-3-one and 5-ene-3 β-ol type steroids in shake flask resulted in the formation of 1, 4-diene-3-one type steroids, the oxidation stopping at this point.
3. Oxidation of 5-ene-3β-ol type steroids was found to result in dehydrogenation of 1-2 position after formation of 4-ene-3-one type intermediate.
4. Tank culture of dehydroepiandrosterone, not possessing a sidechain at 17-position, was found to give a hydroxylated compound, affording, at the same time, 1-2 dehydrogenated oxidation product and a triketone.
5. The route of oxidation of dehydroepiandrosterone by Bacillus pulvifaciens IAM N-19-2 was considered from the foregoing experimental results.
