1961 Volume 7 Issue 3 Pages 161-172
α, ε-Diaminopimelic acid (DAPA) was accumulated by two lysine requiring strains of Micrococcus glutamicus in limited lysine concentration in media. These strains showed neither DAPA decarboxylase nor growth response to DAPA.
A slow growth response to DAPA and DAPA decarboxylase was found in another lysine requiring strain. DAPA accumulation was not recognized by this strain, although trace amounts of DAPA were accumulated in some medium.
Enzyme activity of lysine accumulating system in a lysine-producing strain was inhibited specifically by homoserine or threonine as shown in previous paper. Therefore, inhibition of DAPA accumulation or DAPA decarboxylation will be expected if lysine is synthesized via DAPA. But both homoserine and threonine showed no inhibitory effect either on DAPA synthesis or DAPA decarboxylation while lysine inhibited DAPA synthesis specifically.
From these results, biosynthetic pathway of lysine via DAPA and different pathways of lysine and DAPA were discussed.