BIOGENESIS OF VARIOTIN

I. INCORPORATION OF CH₃¹⁴CO₂H AND ¹⁴CH₃CO₂H INTO VARIOTIN

Abstract

Radioactive acetate, CH₃¹⁴COOH or ¹⁴CH₃COOH, was incorporated into the molecule of variotin with a high yield, when introduced into the culture medium of Paecilomyces varioti. Active carbon atoms were localized in the C₁₃-hydroxy acid moiety of variotin. Distribution of active carbon atoms in the molecule of variotin was suggested to be as follows:
CH₃COOH→
CH₃-CH₂-CH₂-CH=C-CH=C-CH=CH-CH=CH-CONH-(CH₂)₃-COOCH₃
OH CH₃
CH₃COOH→
CH₃-CH₂-CH₂-CH=C-CH=C-CH=CH-CH=CH-CONH-(CH₂)₃-COOCH₃
OH CH₃