INDUCTION AND MORPHOGENESIS OF CULTURED CELLS OF BRYOPHYTES

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  Callus induction was achieved in eight taxa of liverworts, a moss Polytrichum commune and one anthocerote, Anthoceros punctatus. Calluses were formed by culturing spores or thalli on Murashige and Skoog's medium (MS) with 2-4% glucose but without phytohormones, although calluses of P. commune and A. punctatus were induced from cultures on MS medium containing 4% glucose and 10^-6 M 2,4-D. Cell lines in suspension cultures were established from individual callus lines and maintained by subculture at 14 day-intervals. Of these cell lines, that of Pellia endiviifolia (Pe) was examined in detail for its growth characteristics and morphogenesis. The Pe cells grew in the dark as well as in the light and accumulated a large quantity of starch at the stationary phase in suspension culture in darkness.