抄録
SDS electrophoresis and antigen-antibody reactions were carried out in order to investigate the effects of bacterial enzymes isolated from Indian nan and chapati on the decomposition of allergenic proteins in wheat flour.
The salt-soluble and -insoluble fractions of the allergenic proteins were treated with the crude enzymes produced by microorganisms isolated from Nan and chapati. The results of antigen-antibody reactions indicated that only three microorganisms isolated from chapati, 00-IND-016-1, 00-IND-016-3 and 00-IND-016-4, could decompose the allergenic proteins.
These three also exhibited 99% similarity to B. subtilis and B. amyloliquefaciens when analyzed with an API 50 CHB/E medium kit. A phylogenetic tree based on the 16SrRNA sequences showed that the three microorganisms were close to B. methylotrophicus and B. amyloliquefaciens.
