日本家政学会誌
Online ISSN : 1882-0352
Print ISSN : 0913-5227
ISSN-L : 0913-5227
報文
ビシンコニン酸を用いたたんぱく質汚れの簡易評価
谷 道子森田 みゆき
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2018 年 69 巻 4 号 p. 238-242

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  To establish a convenient procedure for analyzing protein soil, we investigated a method for visually evaluating proteins using a bicinchoninic acid assay performed on a solid surface (filter paper). On the filter paper, the color change proceeded in the assay, allowing detection through measurement of surface reflectivity at 560 nm. The assay was performed over ranges of 4℃ to 37℃ and 0 to 100 min to determine optimal assay conditions, which, for a 30 μL sample, were a wavelength for measuring surface reflectivity of 560 nm, a time of 30 min, and a temperature of 25℃. The standard curve showed good linearity at y=-13.65 logx+64.05 (R2= 0.976) over an albumin concentration range of 7.5 to 600 μg. Color differences were observed over an albumin concentration range of 0 to 300 μg. Since the color change proceeded at other temperatures and durations, the assay can be conducted under conditions suited to the environment and purpose.

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