Abstract
The effects of sodium dehydroacetate and sodium lauryl benzene sulfonate on protein biosynthesis in cell free systems of rat liver were examined.
The following results were obtained.
1) Sodium dehydroacetate stimulated slightly the incorporation of 14C-leucine into the total protein by post mitochondrial supernatant at the concentration of 5 and 10 mM, and inhibited by about 90% at the concentration higher than 30 mM. Sodium lauryl benzene sulfonate inhibited the incorporation by about 100% at the concentration higher than 2.5 mM.
2) Sodium dehydroacetate had little effect on 14C-leucine incorporation in microsome and cell sap systems at the concentration of 5 and 15 mM, and inhibited completely 14C-leucine incorporation at the concentration of 50 mM. Sodium lauryl benzene sulfonate inhibited completely 14C-leucine incorporation at the concentration higher than 2.5 mM.
These two additive had effects on the protein biosynthesis by two kinds of cell free systems of rat liver in the similar fashion to the effects observed on the liver slices.
