Effect of Dominant-Negative Expression of L1 Cytoplasmic Domain on the Localization of Brain Ankyrins in Human Neuroblastoma NB-1 Cells

Abstract

Two isoforms of brain ankyrin, 440 kD and 220 kD ankyrin_B, and neuronal cell adhesion molecule L1, which can bind to brain ankyrin, are both expressed in human neuroblastoma NB-1 cells. To examine the potential importance of the interaction between brain ankyrins and L1 in neuronal cell functions, an expression vector encoding a cytoplasmic domain of L1 has been constructed and transfected in NB-1 cells. The results obtained show that the expressed L1 cytoplasmic domain is ineffective in perturbing the interaction between brain ankyrins and L1, possibly because it exists in a monomeric form.