Spectrofluorometric Determination of Uric Acid and Glucose by Use of Fe(III)-Thiacalix[4]arenetetrasulfonate as a Peroxidase Mimic

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The peroxidase-like activity of the Fe³⁺ complex of thiacalix[4]arenetetrasulfonate on a modified anion-exchanger (Fe³⁺-TCAS[4]_A-500) has been applied to the spectrofluorometric determination of uric acid and glucose in connection with uricase and glucoseoxidase (GOD), respectively. Uric acid and glucose were spectrofluorometrically determined by measuring the amounts of H₂O₂ produced through each catalytic reaction by uricase and GOD for uric acid and glucose in a sample solution, respectively. The calibration curves obtained by the Uricase-Fe³⁺-TCAS[4]_A-500 and the GOD-Fe³⁺-TCAS[4]_A-500 methods were linear from 0.5 to 5.0 μg of uric acid in a 1.0 ml sample solution and from 0.5 to 6.0 μg of glucose in a 0.5 ml sample solution, respectively. The methods using Fe³⁺-TCAS[4]_A-500 were applied to the determination of uric acid and glucose in control sera.