Microbial Degradation of Disinfectants. A New Chlorhexidine Degradation Intermediate (CHDI), CHDI-C, Produced by Pseudomonas sp. Strain No. A-3

Abstract

To clarify the degradation pathway of chlorhexidine by a microbe, Pseudomonas sp. Strain No. A-3, the isolation and identification of microbial chlorhexidine degradation products were attempted. A new chlorhexidine degradation intermediate (CHDI), named CHDI-C, was isolated by extraction with ethylacetate, n-butanol, column chromatography using Silica gel, and purified by preparative thin layer chromatography. The chemical structure of this product was examined by infrared, ¹H NMR, ¹³C NMR and fast atom bombardment mass spectra studies. Based on the spectroscopic data, this product was assumed to be a modified compound of chlorhexidine (molecular weight, MW; 530). From the proposed structure, CHDI-C was assumed to be a new chlorhexidine degradation intermediate.