Detection of Antibody and the Antigen Classes in Circulating Immune Complexes Fractionated with the Sucrose Density Gradient Centrifugation Using an F(ab′)₂anti-C3 ELISA

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Circulating immune complexes (CICs) in sera from patients with liver cirrhosis (LC), primary biliary cirrhosis (PBC), hepatitis B type (BCH) and hepatitis not including BCH (nonBCH) were analyzed using F(ab′)₂anti-C3 ELISA, since CICs' functions are intimately associated with the composition and their sizes. Positive percentages for IgG-, IgA- and IgM-CIC were significantly increased in LC, PBC, BCH and nonBCH as compared to healthy specimens. The combination of F(ab′)₂anti-C3 ELISA and sucrose density gradient fractionation allowed us to detect the classes of antibodies and hepatitis B surface antigens in the fractionated CICs from sera of patients with liver diseases. Properties of the fractionized CICs are discussed in the text.