高速液体クロマトグラフィーによる安息香酸ナトリウムカフェインの定量

抄録

Caffeine and sodium benzoate were determined by high performance liquid chromatography with a UV detector by use of stainless column (4.0 mm×0.15 m) packed with Nucleosil ₅C₁₈. Mobile phase was M/30 KH₂PO₄+M/30 Na₂HPO₄·2H₂O-methanol (70 : 30). The sample was dissolved in 20% aqueous methanol. Determination was carried out successfully by using antipyrine as an internal standard and by maintaining the temperature at 40°C. The recovery of sodium benzoate and caffeine from synthetic mixtures were 97.2±1.0% and 99.8±1.1%. This method was applied to three kinds of pharmaceutical preparations. The contents of sodium benzoate and caffeine were 48.4-50.1% and 49.0-49.6%.