キャピラリー電気泳動による健康茶に含まれるセンノシドA, Bの迅速分析
1999 年 45 巻 5 号 p. 293-296
詳細
1999 年 45 巻 5 号 p. 293-296
A method for determinations of sennoside A (SA) and sennoside B (SB) included in "health tea" by capillary electrophoresis (CE) was developed. A 0.02M sodium borate solution adjusted to pH 10.8 was used for the running buffer. SA and SB were detected at UV 260nm. C. V. (%) of migration times for SA and SB were 0.07 and 0.06%, and that of the peak area was 1.3%. The detection limits of SA and SB were 2ppm. As CE has high theoretical plates, the refinement of sample was simplified and the time for analyzing extracts was shortened to about 1/3 of that by HPLC. Six samples of "health tea, " advertised with a likely effect of losing weight, were examined and SA and SB were detected from three of the samples. The total concentrations of SA and SB were 0.11-0.28%.
