唾液腺の組織細胞化学的研究

K⁺ -paranitrophenyl phosphatase, Alkaline phosphatase, Mg²⁺ -ATPase活性の局在について

抄録

Ultrastructural localization of K⁺ -paranitrophenyl phosphatase (K⁺ -NPPase) activity of the Na⁺, K⁺ -ATPase complex, nonspecific alkaline phosphatase (ALPase) and Mg²⁺ -ATPase activities were studied in the rat and human salivary glands.
The results were summarized as follows.
1) K⁺ -NPPase activity: In the acinar cells and the striated ducts the reaction products were present on the cytoplasmic side of the plasma membrane forming basal infoldings, while no reaction was localized to the apical membrane. When ouabain was added to the medium, the reaction was completely absent.
2) AL Pase and Mg²⁺ -ATPase activities: Myoepithelial cells were demonstrated by an ALPase technique in rat, but not in human adult, and by a Mg²⁺ -ATPase technique in rat and human adult. Ultrastructually, the reaction products were associated with the plasma membranes and pinocytotic vesicles of the myoepithelial cells. The salivary glands of the 16 week-old fetus were composed of the primary ducts and the terminal cell clusters. Some flattened cells with bundles of microfilaments were present on the periphery of the terminal cell clusters. The Mg²⁺ -ATPase reaction products were found along all adjoining cell membranes, while no AL Pase reaction was observed.