Journal of Insect Biotechnology and Sericology
Online ISSN : 1884-7978
Print ISSN : 1346-8073
Regular Articles
Biologically active human bone morphogenetic protein 4 fused to collagen-binding domain produced in silkworm-baculovirus expression system
Saki ImaiZhiqing LiKazuhiro IiyamaYoshitaka MiyagawaMasashi ToyodaAkihiro UmezawaHiroaki MonTakahiro KusakabeKaito YoshimuraJae Man Lee
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2013 Volume 82 Issue 2 Pages 2_039-2_044


Human bone morphogenetic protein 4 (BMP4) fused to the collagen-binding domain (CBD) of fibronectin was constructed and expressed in a silkworm-baculovirus expression system. When recombinant BmNPV was injected into the silkworm larvae and harvested after approximately 4 days, 0.22mg/ml (0.1mg/larva) of recombinant CBD-BMP4 was secreted into the silkworm haemolymph. Interestingly, cultured silkworm cells infected with the same recombinant virus could not secrete the recombinant CBD-BMP4 into culture media. The purified rCBD-BMP4 showed Smad- and MAPK-stimulating activities in human UET-13 cells.

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