2021 Volume 90 Issue 3 Pages 3_049-3_054
We developed a new incision sealing method using a paraffin coated paper (PP) instead of melted paraffin (MP) as in the previous method, to improve the ovary transplantation in silkworm, Bombyx mori. Thus, we succeeded in simplifying the incision sealing without reducing the survival rate of recipient larvae. The survival rate of MP, PP, and no sealing (control) was 65.0% (SE 12.6), 75.6% (SE 13.1), and 50.6% (SE 14.0), respectively. Although, among the above three ways, there was no statistical significance, the PP treatment was easier than the MP one. Next, to effectively use cryopreserved resources, we compared three transplantation methods using normal silkworms as recipients and transgenic silkworms as donors: (A) both recipient ovaries were transplanted, (B) only one of the ovaries was transplanted, and the other recipient ovary was removed, and (C) only one of the ovaries was operated like in (B), but the other was still kept in the recipient body. Consequently, the method (C), only one-ovary transplantation, was as high as, or more than, other methods in the effectiveness of the reproduction from the frozen-thawed ovary. Our results suggest that the two methods using PP sealing and one ovary transplantation could be convenient and effective in cryopreservation technology using ovary of silkworm.
