P-02　木質バイオマスからキシロース発酵可能なイオン液体耐性強化酵母の開発

抄録

Woody biomass is attracting attention as an energy source that does not compete with food supply. However, cellulose, one of the main components of woody biomass, is difficult to hydrolysis to glucose, since it exhibits high crystallinity with numerous hydrogen bonds. Use of ionic liquids has been proposed as one of the solutions. Dissolving cellulose in certain ionic liquids reduces crystallinity and facilitates hydrolysis to glucose. On the other hand, wild-type Saccharomyces cerevisiae (S. cerevisiae), which has high ethanol fermentation capacity, has low tolerance to ionic liquids and cannot ferment xylose, one of the sugar components of woody biomass. In this study, we attempted to develop S. cerevisiae that is tolerant to ionic liquid by using our genetically modified xylose-fermenting yeast. We also investigated ethanol production from glucose and xylose using the yeast in the presence of ionic liquids.