1995 Volume 79 Issue 11 Pages 696-701
The laser-induced fluorescence (LIF) method is a useful technique to examine activities in the growing process of plants. In this investigation, a LIF system was devised using an optical multichannel spectrometer, and Ar laser and He-Ne laser lights for excitation. Fluorescence spectra were measured for intact leaves of rice plants with various amounts of nutrients, and rice chlorina mutant plants lacking in chlorophyll b and chlorophyll contents. The chlorophyll fluorescence spectra of rice leaves had two peaks at around 685nm (F II) and 740nm (F I) in relation to photosystem II and I, respectively. These chlorophyll fluorescence spectra showed changes depending on the chlorophyll contents and various amounts of the chlorophyll a/b ratio. The ratios of the two peaks (F I /F II) were proportional to the amount of chlorophyll contained in the rice plant leaves. we conclude that the mesurement of laser induced fluorescence is a powerful method for the study of the activity of plants in the plant factory.