Koganei Cherry Street is located along Tamagawa canal in Tokyo and designated as a national cultural property. However, in recent years decline of cherry trees of this street is concerned about shading from surrounding other trees and incidence of wood-rot disease. We tried to survey the current condition of wood-rot fungi distribution on the cherry trees and to examine the effect of shading on the fungal occurrence. The survey was conducted five times through 2005 to 2007, identifying the fruit bodies of wood-rot fungi and measuring the tree size and the proportion of shaded canopy. In 2007, fruit bodies of wood-rot fungi composed of 38 species in 28 genus were found on 51.5% of 666 cherry trees. Shading did not show significant effect on the occurrence of fruit bodies. On the other hand, tree size had a significant effect and the larger trees suffered form wood-rot disease more frequently than smaller trees.