2016 Volume 30 Issue 4 Pages 165-175
Although recent progress in DNA sequencing technology has revealed the comprehensive profile of the intestinal microbiota, many species are still not-yet-culturable in vitro. However, efforts to isolate and characterize the not-yet-culturable or difficult-to-cultivate bacteria have succeeded to some extent. Here we introduce the classification and characteristics of several bacterial species or groups, including segmented filamentous bacteria (SFB), which were isolated in vitro or in vivo using germ-free animals.