日本応用動物昆虫学会誌
Online ISSN : 1347-6068
Print ISSN : 0021-4914
ISSN-L : 0021-4914
短報
PCR-RFLP法によるネギアザミウマの2生殖型の識別と鳥取県におけるネギアザミウマ産雄性単為生殖型の分布
竹内 亮一𡈽田 聡
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ジャーナル フリー

2011 年 55 巻 4 号 p. 254-257

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To discriminate between arrhenotokous and thelytokous forms of the onion thrips (Thrips tabaci), we developed a simple molecular diagnostic method that uses PCR-RFLP of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Digestion of 490-bp COI amplicons with EcoO109I provided reproductive form-specific RFLP patterns. Using this method, we surveyed the frequencies of the two reproductive forms in 9 Welsh onion fields in Tottori Prefecture. Arrhenotoky dominated in the western area, although 100% of individuals were thelytokous in the eastern area. The COI haplotype of the arrhenotokous individuals was haplotype 1 or 2, which has not previously been found in Japan.

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© 2011 日本応用動物昆虫学会
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