Japanese Journal of Applied Entomology and Zoology
Online ISSN : 1347-6068
Print ISSN : 0021-4914
ISSN-L : 0021-4914
A Method for Rearing the Persimmon Fruit Moth, Stathmopoda masinissa (Lepidoptera:Stathmopodidae) using Cultured Tip Tissue of Japanese Persimmon, Diospyros kaki
Hideshi NakaNatsuko KobayashiKoji TsuchidaHironori Sakurai
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1998 Volume 42 Issue 4 Pages 221-226


The Persimmon Fruit Moth, Stathmopoda masinissa Meyrick is an important pest of Japanese persimmon fruit, but it is difficult to rear under artificial conditions. We report an artificial rearing method using cultured tip tissue of the Japanese persimmon, Diospyros kaki. Significant differences were not found for different sterilizing conditions using corrosive sublimate at egg hatching, but were found at different solution concentrations at pupation and emergence. The decline in hatchability is thought to be due to excess humidity and the lethal effect of the solution on eggs when used for more than the optimum sterilizing period. These results suggest that the best sterilizing condition is a 0.4% solution for 3min. The pupation and emergence rates are highest at 25°C. The theoretical developmental zero point is 12.4°C and the total effective temperature is 523.78 day degrees. The optimum rearing density is two eggs per bottle. Laboratory-reared adults were smaller than wild adults.

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