Japanese Journal of Food Chemistry and Safety
Online ISSN : 2189-6445
Print ISSN : 1341-2094
ISSN-L : 1341-2094
Regular articles
Simultaneous determination of the dipeptide type sweetners in foods by HPLC and LC/MS
Noriri KitanoHanayo TomiokaYoshimi Kitada
Author information

2014 Volume 21 Issue 2 Pages 115-120


A rapid and simple method for the simultaneous determination of the dipeptide type sweetners, those are aspartame (APM), neotame (NE) and advantame (AD), in various foods by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) was developed. The sweetners were extracted from foods with 0.01 mol/L hydrochloric acid in ultrasonic bath, and the extract was loaded on a strata-X-C, cation-exchange and reverse-phase cartridge. The HPLC separation was performed on a Inertsil ODS-4 column (5 μm, 4 mm i.d. × 150 mm) with ultraviolet detection, using 0.01 mol/L phosphate buffer (pH3.5)-acetonitrile as a mobile phase. The LC/MS separation was performed on a InertSustain C18 column (3 μm, 2.1 mm i.d. × 50 mm) with a mobile phase of 0.01 mol/L ammonium formate-formic acid buffer (pH3.5)-methanol, and MS detection with positive ion electrospray ionization. In HPLC, the quantification limit of three sweetners was 0.001 g/kg. In LC/MS, the limit of APM was 0.005 g/kg, and that of AD and NE was 0.001 g/kg. The mean recoveries from foods fortified at the levels of 0.02 and 0.20 g/kg were 87.8-106.3% in HPLC, and 64.0-128.9% in LC/MS.

Information related to the author
© 2014 Japanese Society of Food Chemistry
Previous article Next article