Polyphenol content in leaf blades of five strawberry cultivars (Fragaria x ananassa Duch. cv.: 'Toyonoka', 'Hinomine', 'Nyohou', 'Harunoka', and 'Haruyoi') was determined by HPLC. Among 7 polyphenols, i.e., (+)-catechin (1), (+)-afzelechin-(4α-8)-(+)-catechin (2), procyanidin B3 (3), procyanidin B6 (4), pedunculagin (5), (+)-taxifolin 3-O-α-L-arabinofranoside (6) and (+)-taxifolin (7), the major constituents accumulated were 1 and 3. In all cultivars, the contents (% dry weight) of 1 and 3 were in the ratio of almost 1:1 at every season, which levels changed between the range of 0.1-1% during a year.