Abstract
The newly notified analytical method for aflatoxins was established by improving the previous reports. An inter-laboratory validation studies were conducted to validate the analytical method. Aflatoxins B1, G1, G2 and B2 spiked into peanuts, pistachio nuts, corn, red pepper and nutmeg at the level of 10 ng/g were analyzed in replicates in 5 laboratories. Mean recoveries of aflatoxin B1 were 77.9〜92.7% and those of aflatoxins G1, G2 and B2 were 81.5〜92.5%, 84.7〜82.2% and 83.7〜89.1%, respectively. Repeatability relative standard deviations of aflatoxins B1, G1, G2 and B2 in five crops were in the ranges of 2.9〜7.3%, 2.1〜6.5%, 1.3〜5.2% and 1.9〜4.6%, respectively. Reproducibility relative standard deviations of aflatoxins B1, G1, G2 and B2 in five crops were in the ranges of 6.5〜10.1%, 2.5〜14.2%, 6.5〜10.1% and 5.3〜10.6%, respectively. These results suggested that the proposed method is reliable and reproducible for the inspection of aflatoxin levels in peanuts, pistachio nuts, corn, red pepper and nutmeg.
