1990 Volume 72 Issue 1 Pages 17-22
A new bacterial disease with gall formation on either trunks or twigs of himeyuzuriha (Daphniphyllum teijsmanni Z.) trees was found, growing in Okinawa, Kagoshima, Miyazaki, and Kochi Prefectures, Japan. Galls with rough, irregular surfaces are light- to dark-brown and are one to several centimeter in diameter. A pathogenic bacterium isolated from these galls was a gram-negative aerobic rod, 2.1×0.6 μm, with 1 to 10 polar flagella, which metabolized glucose oxidatively. LOPAT tests were - or +, - -, - and +. Negative results were obtained with tests such as the production of H2S, hydrolysis of starch, nitrate reduction, indole production, gelatin liquefaction, gluconate oxidation and growth at 41 and 4°C. Positive results were obtained with tests such as the production of fluorescent pigment and reducing substance from sucrose, and hydrolysis of Tween 80. Glucose, sucrose, L-arabinose, succinate, and D-, L-, and meso-tartrate were utilized as sole sources of carbon. L-rhamnose, glycogen, lactate, L-arginine, L-valine, and β-alanine were not utilized as sole sources of carbon. This bacterium was pathogenic only on D. teijsmanni, but not on 70 other species of trees belonging to 39 families. From these results, the himeyuzuriha pathogen was identified as a new pathovar of Pseudomonas syringae VAN HALL. The name of Pseudomonas syringae pv, daphniphylli pv. nov. was proposed, and the strain DAT 1 (=ATCC 49211, NCPPB 3617, ICMP 9757) was designated as the pathotype strain.