The present investigation was undertaken to determine the effects of glutamic or aspartic acids on ammonia metabolism in vitro in the separated brain and liver from animals exposed to noise or cold.
The adult male rats were exposed to noise or cold, as previously mentined. Thereafter, the animals were decapitated, and the brain and liver were immediately removed. These tissues were homogenized in Tris buffer (pH 7.4) with glass homogenizers in the cold. The mixture of 2 parts of each of these homogenates and 1 part of each of these amino acids solutions was incubated at 37C for one hour. And then, the ammonia content of the reaction mixture was measured.
Under the control and the cold conditions, the addition of glutamic or aspartic acid to the homogenate of brain or liver caused a decrease in the ammonia content, but in the noise condition, caused an increase.