2倍体および3倍体ギンブナの赤血球径の比較
1977 年 24 巻 2 号 p. 135-140
詳細
1977 年 24 巻 2 号 p. 135-140
As regards size of erythrocytes, the senior author reported two types of the ginbuna Carassius auratus langsdorfi, one with smaller erythrocytes and the other with larger erythrocytes, from Naka River, Shizuoka Prefecture (Sezaki, 1974).In the present study, the relationship between the erythrocyte size and somatic chromosome number was examined.Blood smear preparations were made by ordinary method with Giemsa's solution and chromosome preparations were performed by the same method previously described (Kobayasiet al., 1970).
Among 16 fish examined, somatic chromosome number of 4 males and 2 females was 100, and that of 10 females was 156.In the diploid fish the major diameter of erythrocytes was 14.17±0.47 μ, and that of nuclei 5.76±0.30 μ, whereas in the triploid fish the major diameter of erythrocytes was 17.96±0.51 μ, and that of nuclei was 7.97±0.34 μ. The ratio of the area of the triploid erythrocytes to that of diploid ones was 1.30, and that of nuclei was 1.34. The results show that it is easy to distinguish the triploid ginbuna from the diploid one by the examination of the erythrocyte size.
