Fluorescent Measurement of Membrane Transport Using Artificial Lipid Bilayer Microchambers

Abstract

Artificial lipid bilayers structurally mimic cell membrane. Such the artificial lipid bilayers have been used for analyzing membrane transport by incorporating transporters in the artificial lipid bilayers. The membrane transport analysis using the artificial lipid bilayer is advantageous in the sense that less amount of off-target molecules exists in the system compared with the method using the actual cell membrane. That feature makes the measurement highly sensitive and reliable. In this report, the author introduces one of the methods to analyze membrane transport using the artificial lipid bilayers, focusing on fluorescent measurement of membrane transport using artificial lipid bilayer microchambers.