Detection of Aflatoxins in Autopsied Materials from a Patient Infected with Aspergillus flavus

抄録

We isolated two strains of Aspergillus flavus from a lung lesion and a skin lesion at autopsy from a patient with acute myelogenous leukemia complicated with fungal infection.
An attempt was made to detect aflatoxins in culture filtrates of those isolates and the tissue extract of the lung lesion through the techniques of thin-layer chromatography (TLC), densitometry and high-performance liquid chromatography (HPLC). Aflatoxins B₁, B₂ and M₁ were demonstrated in all of these materials qualitatively and quantitatively. The concentrations of aflatoxins in the cultures of the isolates and in the lung lesion extract determined by HPLC were aflatoxin B₁: 11.715μg/ml (lung isolate), 21.383μg/ml (skin isolate), 0.635μg/g (lung extract), aflatoxin B₂: 0.341μg/ml (lung isolate), 0.577μg/ml (skin isolate), 0.0273μg/g (lung extract) and aflatoxin M₁: 0.277μg/ml (lung isolate), 0.491μg/ml (skin isolate), 0.0525μg/g (lung extract), respectively.
B₁, known as the most toxic among the aflatoxin group, showed the highest concentration through these experiments.
This case may be considered as the first to detect aflatoxins in autopsied materials associated with A. flavus infection.