Highly-concentrated glucose aqueous solution protects hiratake (Pleurotus ostreatus) mycelia during cryopreservation at −20℃

Abstract

In this study, we placed the mycelial disks of six strains of hiratake grown on agar plates into a 40％ (w/w) glucose solution, used as a cryoprotectant, and incubated them at 7℃ or 25℃ for 0 (control)－24 h before freezing. The survival rates of two strains (ANCT-15001 and Po 89-1) were the highest when the discs had been incubated at 25℃ for 24 h and were 100％ even after storage at －20℃ for 10 wk. Although mycelial growth on PDA plates was delayed after cryopreservation for 10 wk, no change in fruiting ability of the two strains was observed.