凍結乾燥軟骨に関する研究

凍結乾燥軟骨の作製法

抄録

Auricular and rib cartilages were taken from immature rabbits of 40 days post partum. Lyophilized cartilages were prepared by rapid freezing at -50°C with vacuum degree of 0.03 Torr.
The results of this study on lyophilized cartilages are summarized as follows:
1) Lyop ilized cartilages showed 10% reduction as compared to fresh cartilages. However, lyophilized cartilages recovered almost the same size and macroscopic findings as those fresh cartilages when immersed for 30 min in a phisiological saline solution.
2) Histopathologically, lyophilized cartilages maintained their intrinsic cartilage structure.
3) The residual moisture was 4.83% in a 5×5mm piece and 4.90% in a 5×10mm piece of the lyophilized auricular cartilage, while it was 4.32% and 4.50% in 5mm and 10mm pieces of the lyophilized rib cartilage respectively.
4) Living cells were not observed in the tissue culture of lyophilized cartilages.