Japanese Journal of Oral and Maxillofacial Surgery
Online ISSN : 2186-1579
Print ISSN : 0021-5163
ISSN-L : 0021-5163
Retinoic acid, EGF and cholera toxin stimulate colony formation of the epithelial cells from rabbit oral mucosa
Katsuyoshi IKESHIMAShiro KASAITakashi TERASAWAHiroshi TAKAI
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1985 Volume 31 Issue 6 Pages 1362-1369

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Abstract

We examined the effect of retinoic acid (RA), EGF and cholera toxin on the colony formation in the primary culture of the epithelial cells taken from the rabbit oral mucosa and the following results were obtained:
1. RA showed the most stimulatory effect on squamous colony formation at the concentration of 10-6-10-7 M. RA also stimulated in part of adenocytoid colony formation by the mucous metamorphosis.
2. The cultures containing EGF revealed about twohold to threehold increase in the colony numbers as compared with the controls. The optimal concentration for the growth of epithelial cell colonies was at around 1-10ng/ml of EGF. Inhibitory effect for the colony formation was observed at 100 ng/ml. Furthermore, it is to be noted that the induction of mucous metamorphosis by EGF was not seen.
3. The enhancing effect of CT on the colony formation was observed in the number of colonies twice as much as that of the controls at the concentration of 10-8-10-9 M.
4. It was clearly observed that synergetic effect in the combination of RA and EGF or EGF and CT revealed about two fold increase in the number of colonies as compared with a single addition of these reagents to the cultures.
5. RA or the combination of RA and CT or RA and EGF did not stimulate the formation of original cell colonies which consisted of small polygonal cells. However, original cell colony formation was significantly stimulated by the addition of EGF.

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