A human oral cancer/nude mice system has been established for an anticancer agent screening system, which is expected to have higher predictability of clinical effect. In this preliminary report, the method of successive transplantation of human cancer in nude mice and the histological characteristics of the tumor were reported.
1) 8-to 10-week-old male BALB/cA-nu mice kept in vinyl isolater were used.
2) Human cancer in this experiment was derived from an oral floor tumor histologically diagnosed as squamous cell carcinoma in a 58-year-old man.
3) The tumor tissue which was minced in sterilized penicilline-streptomycin PBS (-) solution (PC: 100 u/ml, SM: 100γ/ml) was sectioned in small pieces approximately 1mm 3 in volume. 2 to 3 pieces were injected into subcutaneous tissue of the back of each animal.
4) After the fifth generation of serial transplantation, the tumors became stable, showed stable growth curves and maintained the histological features of moderately differentiated squamous cell carcinomas.