Establishment of transplantable human osteosarcoma from mandible into nude mouse and anticarcinoma activity of Rhodamine-123

Abstract

Osteosarcoma is high malignant neoplasm and the prognosis is poor. We established transplantable strains of nude mouse derived from human osteosarcoma originating in mandible (HOSMN-1) and experimented on anticarcinoma activity of Rhodamine-123, a mitochondrial specific dye, and the following results were obtained.
(1) Histologically, HOSMN-1 consisted of atypical spindle, polygonal cells and bizarre multinucleated giant cells, and collagen fibers were also identified. In some places, storiform like pattern was revealed.
(2) Both alkaline phosphatase (ALP) and acid phosphatase (ACP) stains showed positive granules in the tumor cells.
(3) In chromosomal analysis, it was confirmed that HOSMN-1 was of the human origin. We have maintained the tumor cells for about 2 years for 10 serial passages, but up to date, no malignant osteoid was identified. HOSMN-1 hadn't revealed any differentiation or dis-differentiation.
(4) On anticarcinoma activity of Rhodamine-123, a group administered 15 mg/kg × 4 times (i.m.) had significantly showed a growth inhibition effect in comparison with a control group, and the disappearance of tumor in 30% was observed.
(5) Ultrastructurelly, in a group administered Rhodamine-123, especially, mitochondrial expansion, distortion and disappearance of crista were markedly seen. These findings supported the contention that Rhodamine-123 is a specific mitochondrial dye.