実験的ハムスター舌癌組織における神経線維の動態

抗NFP, S-100蛋白抗体を用いた免疫組織化学的研究

抄録

Innervation of carcinomas induced by application of 9, 10-dimethy1-1, 2-benzanthracene (DMBA) was investigated in the hamster tongue by using immunohistochemistry concerning neurofilament protein (NFP) and glia specific S-100 protein.
The tumor induced by application of DMBA was a well-differentiated squamous cell carcinoma having cord-like invasion of the underlying fibrcus tissue and muscle layer.
The characteristic feature of the innervation was the extreme scarcity of NFP-and S-100 pro tein-immunopositive neural elements recognized in the stroma and around blood vessels of the carcinomas.
A few NFP-positive neural elements which were observed in the carcinoma invading zone were very fine and also found not to bear any relation to blood vesseles nor tumor nests.
Unlike calcitonin gene-related peptide-positive nerves, a dense distribution of S-100 protein-positive neural elements characteristic of arterioles in the surrounding muscle layer was also not seen around arterioles abundant in the carcinoma invading zone.
The results indicate a high pain threshold and the absence of autoregulatory mechanism in the blood vessels of the carcinoma tissue.