培養口腔癌細胞 (KB, IT-2) における血管新生因子の検討

抄録

The progressive and continuous growth of malignant tumor tissue is possible only after induction of a vascular network into the tumor tissue from the surrounding tissue. Some chemical substances produced by cancer cells are known to stimulate the angiogenic process in the tumor tissue in either a paracrine or autocrine manner. The authors investigated the angiogenic process of cultured oral carcinoma cells using an in vitro experimental model. The cancer cell lines examined were KB cells and a cell line established from the metastatic lymph node of mucoepidermoid carcinoma, designated IT-2. In vitro angiogenic activities in conditioned media (CM) of KB and IT-2 were enhanced 7.3 and 6.0 times as compared with non-conditioned medium. In addition, the enhancing effect of CM was markedly diminished by pretreatment of CM with anti-b FGF or anti-VEGF Ig Gs. These results suggest that the angiogenic activities of KB and IT-2 depend on both b FGF and VEGF.