顎嚢胞壁組織におけるsuperoxide dismutaseの酵素活性ならびに局在性に関する研究

抄録

To clarify the role of superoxide dismutase (SOD) in the tissue of jaw-cyst walls, the enzyme activity and the localization of SOD were investigated using various kinds of cyst walls that were surgically removed as samples. The enzyme activity of copper-zinc (Cu, Zn) and manganese (Mn) SOD were determined by an MCLA-dependent chemiluminescence method and the localization of Cu, Zn-SOD was studied by an immunohistochemical staining method, as compared with the normal gingiva as control.
The mean activities of both Cu, Zu and Mn SOD of the cyst walls were lower than that of control. On the other hand, Mn-SOD activity was slightly higher than Cu, Zn-SOD activity. Cu, Zn-SOD immunoreactive staining was mainly observed in the cyst-wall epithelium. The intensity of Cu, Zn-SOD staining was markedly increased in the squamous cells, especialy in the hyperkeratinzed squamous cells of the cyst wall. Moreover, vascular endothelial cells and inflammatory cells in the connective tissue showed positive SOD staining according to the degree of inflammatory changes in the cyst wall. These findings suggested that SOD in the jaw cyst wall might provide protection against tissue injury caused by oxidative stress.