2007 Volume 53 Issue 10 Pages 580-587
To elucidate the effects of a fibroblast inhibitor (tranilast), we investigated the proliferation, invasion, and metastasis of oral squamous cell carcinoma (OSC-19) cells. The effects of tranilast on tumor growth, lymph node metastases, microvessel density, and the proliferating cell nuclear antigen (PCNA) labeling index of oral squamous cell carcinoma implanted into the tongue of nude mice were evaluated. Moreover, we investigated the invasion of OSC-19 cells into fibroblast-embedded collagen gels. Tumor growth and the incidence of cervical lymph node metastases were significantly suppressed by treatment with tranilast. The amount of fibrous tissue, microvessel density, and the PCNA labeling index of the tumors were also significantly reduced. In an invasion model, OSC-19 cells invaded collagen gels in control culture medium, but did not invade collagen gels in culture medium with tranilast. Administration of a fibroblast inhibitor may well be clinically effective for the treatment of oral squamous cell carcinoma.