To reveal the relation with swelling and fatigue on lower leg, 19 legs of working women in the morning and in the evening were measured by physical and physiological measurements. The effects of swelling were confirmed by the volume of lower leg and the ratio of extracellular water. Similarly the effects of fatigue on lower legs were confirmed by the muscle stiffness, but not confirmed by the electromyogram and mechanomyogram on voluntary muscle construction. It suggested that the relation with fatigue and swelling on lower legs were not clearly confirmed by using the voluntary muscle construction method.