Abstract
There are many reports about what kinds of growth factors act on stem cell in hematopoiesis, but only a few of them refer to metabolic dynamics in cell and environment after the action of growth factors. The purpose of this study was investigation of dynamics of fat metabolism on erythropoiesis. Rats were used for this studyin vivoandin vitro. They were established as a hemolytic anemia model in our department. (1) Variation of fatsin vivo: Most of fats in serum, supernatant of bone marrow and bone marrow cell decreased in the early stage and increased in the intermediate stage on erythropoiesis. But only phospholipid in supernatant of bone marrow and PI + PS in bone marrow cell increased from the early stage. And the recovery of unsaturated fatty acids in bone marrow cell was slower than that of saturated fatty acids. (2) Variation of fatsin vitro: Erythropoietin produced the decrease of archidonic acid in bone marrow cell. The concentration of IP3in bone marrow cell at the 4th day was higher than at the first day. But Epo did not produce the increase of IP3 in bone marrow cell. These data suggest the possibility that archidonic acid and phospholipids play a role in hematopoiesis. IP3 might not be concerned with the intracellular response for the maturation of erythroid progenitor cell by erythropoietin.
