医療薬学
Online ISSN : 1882-1499
Print ISSN : 1346-342X
ISSN-L : 1346-342X
一般論文
Tissue Distribution of Intravenously Administered Insoluble Microparticles: Report II
Takuya SugaharaMasaharu KotaniKeiji KuramotoKouichi AraiTadashi ShiraishiTohru Shoji
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ジャーナル フリー

2012 年 38 巻 9 号 p. 559-567

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In this study, we investigated the in vivo distribution of insoluble fluorescent microparticles administered to rats through the central vein. We previously reported that the IMs increased by mixed operation of some injections. The number of 10-25 μm insoluble microparticles was 41 ± 1 /mL. Therefore, the daily 10-μm fluorescent microparticle dose was set at 2000/day for the nutritional solution dose of 50 mL/day required to maintain the rats. The fluorescent microparticles were administered by bolus injection into the central vein on Days 1, 2, and 3. A fluorescence microscope was used to assess frozen thin sections of each organ that were prepared on Day 4 and urine samples. A large number of fluorescent microparticles were found in the lung tissue, with 1-5 particles in each of the 20-μm thick and 25-mm2 area sections. In 20-μm thick and 50-mm2 area kidney tissue sections, the number of observed fluorescent microparticles ranged from 0.1 to 0.2. No fluorescent microparticles were detected in tissue samples from the veins, cerebrum, cerebellum, heart, or liver. The total number of fluorescent microparticles excreted in the urine was 283.4 ± 113.0, which only accounted for approximately 4.7% of the dose administered over the 3-day period. These findings suggest that 10 μm insoluble microparticles administered intravenously, accumulate mainly in the lungs and kidneys and are excreted at low levels in the urine.

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© 2012 Japanese Society of Pharmaceutical Health Care and Sciences
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