The bactericidal activities of 4 disinfectants (chlorhexidine gluconate, benzethonium chloride, benzalkonium chloride and povidone iodine) and 2 antibiotics (polymyxin B and amphotericin B) were evaluated for organisms isolated clinically from urine (S. aureus, E. faecalis, E. coli, K. pneumoniae, S. marcescens, P. mirabilis, P. aeruginosa and C. albicans), using E. Coli NIHJ JC-2 as a standard strain. Povidone-iodine was remarkably effective for all strains, but chlorhexidine gluconate was slightly effective.
The bactericidal activities of potassium permanganate, benzalkonium chloride, chlorhexidine gluconate, alkyldiaminoethylglycine hydrochloride, acrinol, povidone iodine and polymyxin B against E. coli NIHJ JC-2 were also investigated in the presence of human blood albumin. Povidoneiodine, potassium permanganate, benzalkonium chloride, alkyldiaminoethylglycine hydrochloride, acrinol and polymyxin B had a tendency for decreasing in the bactericidal activity. On the other hand, the bactericidal activities of benzethonium chloride and chlorhexidine gluconate were not changed. Relating to histopathological changes of the rat urinary bladder, interstitial edema was observed when the rat was treated with chlorhexidine gluconate, povidoneiodine or alkyldiaminoethylglycin hydrochloride, but there was little change in the case of treatment with benzalkonium chloride.