N, N-ジエチル-p-フェニレンジアミンとキサンツレン酸による過酸化水素定量およびその応用としての血清シュウ酸測定

抄録

A new colorimetric procedure is described for the determination of hydrogen peroxide.This method is based on oxidation reaction between N, N-diethyl-p-phenylenediamine and xanthurenic acid in the presence of peroxidase to form a quinonoid dye.The green chromophore, which is formed in the condition of pH 3.0-4.4, has an absorption peak at 750nm without serum proteins. This system can be utilized for the determination of serum oxalate using oxalate oxidase with the pre-treatment of deproteinization.The determination of serum oxalate can be completed within 15min, and results are linearly related to oxalate concentrations up to 500μM. This procedure is rapid, simple, and precise.